R&D Projects & Partners
Since the start of IsoLife, we have continuously been investing in the development of new applications with uniformly (>97 atom%), intrinsically labelled whole plants or plant components, in co-operation with many enthusiastic partners – sometimes united in consortia such as BASIS and JESIUM – resulting in the following projects.
Background information about techniques and isotope facilities can be found under Useful links.
2026-2029 (in prep)
31. New 13C-labelled food crops for health & nutrition research
2023-2027 (granted)
30. Investigating crystallinity changes by two-dimensional 13C–13C solid-state NMR spectroscopy
Public output:
Doi Y, K Daicho, R Kusumi, J Ashida, A Gorissen, R de Visser, S Fujisawa, T Saito. 2026.
Molecular mechanism underlying the crystallinity changes of cellulose upon fibrillation and reassembly revealed via two-dimensional solid-state NMR.
Biomacromolecules 27: 1905−1914.
2022-2027 (granted)
29. Unravelling the role of soil microbial communities in the formation and stabilisation of soil organic matter
2020-2024 (granted)
28. Lignin as intrinsic tracer for gastrointestinal transit behavior and feed digestion (LiFeTracer)
Public output:
Veersma RJ, C Lannuzel, WJJ Gerrits, S de Vries, G van Erven, MA Kabel. 2025.
Lignin in fibrous feed as an internal digestibility and transit marker in pigs.
ACS Agricultural Science & Technology 5: 1025−1033.
Veersma RJ, G van Erven, C Lannuzel, S de Vries, MA Kabel. 2024.
Quantitative 13C-IS pyrolysis-GC-MS lignin analysis: Overcoming matrix effects in animal feed and faeces.
Journal of Analytical and Applied Pyrolysis 183: 106802.
Lannuzel C, RJ Veersma, N Wever, G van Erven, MA Kabel, WJJ Gerrits, S de Vries. 2024.
Particle size of insoluble fibres and gelation of soluble fibres influence digesta passage rate throughout the gastrointestinal tract of finishing pigs.
Animal 18: 101175.
2019-2023 (granted)
27. The fate of nutrients captured in the dark films of mushroom compost
Public output:
Duran K, M Kohlstedt, G van Erven, CE Klostermann, AHP America, E Bakx, JJP Baars, A Gorissen, R de Visser, RP de Vries, C Wittmann, RNJ Comans, TW Kuyper, MA Kabel. 2024.
From 13C-lignin to 13C-mycelium: Agaricus bisporus uses polymeric lignin as a carbon source.
Science Advances 10: eadl3419.
Duran K. 2023.
From plant to fungal biomass: Agaricus bisporus can utilise lignin and humic substances.
PhD Thesis. Wageningen University.
Duran K, J Miebach, G van Erven, JJP Baars, RNJ Comans, TW Kuyper, MA Kabel. 2023.
Oxidation-driven lignin removal by Agaricus bisporus from wheat straw based compost at industrial scale.
International Journal of Biological Macromolecules 246: 125575.
Duran K, J Magnin, AHP America, M Peng, R Hilgers, RP de Vries, JJP Baars, WJH van Berkel, TW Kuyper, MA Kabel. 2023.
The secretome of Agaricus bisporus: Temporal dynamics of plant polysaccharides and lignin degradation.
iScience 26: 107087.
Duran K, M van den Dikkenberg, G van Erven, JJP Baars, RNJ Comans, TW Kuyper, MA Kabel. 2022.
Microbial lignin degradation in an industrial composting environment.
Bioresource Technology Reports 17: 100911.
2017-2019 (granted)
26. Development of novel 13C-labelled algae
2016-2018 (granted)
25. Efficient co-extraction of 13C-labelled phytochemicals
Bertran-Llorens S, F Perondi, AL Slama de Freitas, J Chen, G van Erven, PJ Deuss. 2024.
Supercritical CO2 as effective wheat straw pretreatment for subsequent mild fractionation strategies.
Chemical Engineering Journal 497: 154491.
Van Erven G. 2020.
Quantitative mapping of lignin – Comprehensive insight into fungal delignification of plant biomass.
PhD Thesis. Wageningen University.
Van Erven G, R de Visser, P de Waard, WJH van Berkel, MA Kabel. 2019.
Uniformly 13C labeled lignin internal standards for quantitative pyrolysis−GC−MS analysis of grass and wood.
ACS Sustainable Chemistry & Engineering 7: 20070−20076.
Van Erven G, R de Visser, DWH Merkx, W Strolenberg, P de Gijsel, H Gruppen, MA Kabel. 2017.
Quantification of lignin and its structural features in plant biomass using 13C lignin as internal standard for pyrolysis-GC-SIM-MS.
Analytical Chemistry 89: 10907–10916.
2015-2016 (granted)
24. Feasibility of 13C-labelled plant extracts as internal standards in metabolomics studies in Life Sciences and Health. Enabling Technology Hotel Project. This project was made possible by
2014-2018 (granted)
23. Optimizing early-life dietary carbohydrates to improve later-life metabolic health
Public output:
Fernández-Calleja JMS, LMS Bouwman, HJM Swarts, A Oosting, J Keijer, EM van Schothorst. 2019.
Extended indirect calorimetry with isotopic CO2 sensors for prolonged and continuous quantification of exogenous vs. total substrate oxidation in mice.
NATURE – Scientific Reports 9: 11507.
Fernández-Calleja JMS, LMS Bouwman, HJM Swarts, N Billecke, A Oosting, J Keijer, EM van Schothorst. 2019.
A lowly digestible-starch diet after weaning enhances exogenous glucose oxidation rate in female, but not in male, mice.
Nutrients 11: 2242.
2013-2018 (granted)
21. Chitin-degrading microbiome of an agricultural soil
Public output:
Wieczorek AS, O Schmidt, A Chatzinotas, M von Bergen, A Gorissen, S Kolb. 2019.
Ecological functions of agricultural soil bacteria and microeukaryotes in chitin degradation – a case study.
Frontiers in Microbiology 10: 1293.
Wieczorek A. 2018.
Trophic interactions of a chitin-degrading microbiome of an aerated agricultural soil.
PhD Thesis. University of Bayreuth.
Wieczorek AS, N Jehmlich, M von Bergen, S Ferdi, A Chatzinotas, A Gorissen, HL Drake, HJM Op den Camp, S Kolb. 2015.
Chitin degradation by a soil microbiome.
Poster presentation Gordon Research Conference on Applied and Environmental Microbiology, July 11-12, South Hadley, Massachusetts.
2013-2015 (granted)
21. Authentication of flower bulbs
Public output:
Van Ruth SM, R de Visser. 2015.
Provenancing flower bulbs by analytical fingerprinting: Convallaria majalis.
Agriculture 5: 17-29.
2012-2017 (granted)
20. NMR spectroscopy of intact, uniformly 13C-labeled seeds
Public output:
Fortier-McGill B, RD Majumdar, L Lam, R Soong, YL Mobarhan, A Sutrisno, R de Visser, MJ Simpson, H Wheeler, M Campbell, A Gorissen, AJ Simpson. 2017.
Comprehensive multiphase (CMP) NMR monitoring of the structural changes and molecular flux within a growing seed.
Journal of Agricultural and Food Chemistry: 65: 6779–6788.
Lam L, R Soong, A Sutrisno, R de Visser, MJ Simpson, H Wheeler, M Campbell, WE Maas, M Fey, A Gorissen, H Hutchins, B Andrew, JO Struppe, S Krishnamurthy, R Kumar, M Monette, H Stronks, A Hume, AJ Simpson. 2014.
Comprehensive multiphase NMR spectroscopy of intact 13C labeled seeds.
Journal of Agricultural and Food Chemistry 62: 407-415.
2012-2016 (granted)
19. Protein bio-availability, gut barrier function and intestinal immunity
2010-2013 (granted)
18. Further development of advanced features of the plant labelling facility
17. Research on the development of health claims for food crops using 13C-labelling
Public output:
Gorissen A, NU Kraut, R de Visser, M de Vries, H Roelofsen, RJ Vonk. 2011. (see Applications)
No de novo sulforaphane biosynthesis in broccoli seedlings.
Food Chemistry 127: 192-196.
2009-2015 (granted)
16. Development of an advanced 13C-trace gas detector
15. 13C-Oligosaccharide internal standards in clinical chemistry
2008 (granted)
14. Stable Isotope Probing with 13C in microbial ecology
13. Hemp oil and 13C-Internal Standards
12. Bioavailability of healthy components in 13C-Vegetables
11. Further development of advanced features of the plant labelling facility
10. Bioavailability of different 13C-Starches
2007 (granted)
9. Research on 13C-Internal Standards for metabolomics applications
8. Development of advanced features of the plant labelling facility
7. Bioavailability of healthy components in 13C-Vegetables
6. Bioavailability of different 13C-Starches
2006 (granted)
5. Method development 13C-Assimilate distribution in greenhouse cucumber
4. Project on plant methane emission
Public output:
Dueck TA, R. de Visser, H Poorter, S Persijn, A Gorissen, W de Visser, A Schapendonk, J Verhagen, J Snel, FJM. Harren, AKY Ngai, F Verstappen, H Bouwmeester, LACJ Voesenek, A van der Werf. 2007.
No evidence for substantial aerobic methane emission by terrestrial plants: A 13C-labelling approach.
New Phytologist 175: 29-35.
3. Development of 13C-Internal Standard methods for tomato and potato
2. Method development for purification of 13C-Phytosterols and 13C-Flavonoids
1. Short Chain Fatty Acids form 13C-Starch in relation to colon cancer
Partners
