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  • © 2023

Microbial Environmental Genomics (MEG)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2605)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Metabarcoding Approaches for Soil Eukaryotes, Protists, and Microfauna

    • Guillaume Lentendu, Enrique Lara, Stefan Geisen
    Pages 1-16
  3. Metabarcoding of the Three Domains of Life in Aquatic Saline Ecosystems

    • Delphine Melayah, Zélia Bontemps, Maxime Bruto, Agnès Nguyen, Philippe Oger, Mylène Hugoni
    Pages 17-35
  4. Sample Preparation for Fungal Community Analysis by High-Throughput Sequencing of Barcode Amplicons

    • Karina E. Clemmensen, Katarina Ihrmark, Mikael Brandström Durling, Björn D. Lindahl
    Pages 37-64
  5. Characterization of Seed Mycobiota Using Culture-Dependent and Culture-Independent Approaches

    • Gillian E. Bergmann, Sabrina J. Heitmann, Posy E. Busby, Johan H. J. Leveau
    Pages 65-78
  6. Tissue Cultivation, Preparation, and Extraction of High Molecular Weight DNA for Single-Molecule Genome Sequencing of Plant-Associated Fungi

    • Laure Fauchery, Maxim Koriabine, Lillian P. Moore, Yuko Yoshinaga, Kerrie Barry, Annegret Kohler et al.
    Pages 79-102
  7. Analysis of Ancient Microbial DNA

    • Olivier Gorgé, E. Andrew Bennett, Diyendo Massilani, Julien Daligault, Eva-Maria Geigl, Thierry Grange
    Pages 103-131
  8. Investigating the Bacterial and Fungal Communities Involved in Dead Biomass Degradation in Forest Soils

    • Martina Štursová, Ruben López-Mondéjar, Petr Baldrian
    Pages 157-168
  9. Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions

    • Amandine Lê Van, Marie Duhamel, Achim Quaiser, Philippe Vandenkoornhuyse
    Pages 169-186
  10. Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis

    • Sophie Comtet-Marre, Oshma Chakoory, Pierre Peyret
    Pages 187-208
  11. Analyzing Prokaryotic Transcriptomics in the Light of Genome Data with the MicroScope Platform

    • David Roche, Alexandra Calteau, David Vallenet
    Pages 241-270
  12. MycoCosm, the JGI’s Fungal Genome Portal for Comparative Genomic and Multiomics Data Analyses

    • Steven R. Ahrendt, Stephen J. Mondo, Sajeet Haridas, Igor V. Grigoriev
    Pages 271-291
  13. Investigating Endobacteria that Thrive Within Mucoromycota

    • Alessandro Desirò, Yusuke Takashima, Gregory Bonito, Tomoyasu Nishizawa, Kazuhiko Narisawa, Paola Bonfante
    Pages 293-323
  14. Confocal Laser Scanning Microscopy Approach to Investigate Plant–Fungal Interactions

    • F. Fracchia, V. Basso, F. Guinet, C. Veneault-Fourrey, Aurélie Deveau
    Pages 325-335
  15. Back Matter

    Pages 363-365

About this book

This volume guides researchers on how to characterize, image rare, and  hitherto unknown taxa and their interactions, to identify new functions and biomolecules and to understand how environmental changes condition the activity and the response of the organisms living with us and in our environment.

Chapters cover different organism types (i.e., archaea, bacteria, fungi, protest, microfauna and microeukaryotes) and propose detailed protocols to produce high quality DNA, to analyse active microbial communities directly involved in complex interactions or processes through stable isotope probing, to identify and characterize of new functional genes, to image in situ interactions and to apply bioinformatics analysis tools to complex metagenomic or RNAseq sequence data. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Microbial Environmental Genomics (MEG): Methods and Protocols, Second Edition aims to serve as a primary research reference for researchers in microbiology working to in the expanding field of molecular ecology and environmental genomics.

Editors and Affiliations

  • Tree-Microbe Interact (IAM); Biogeochem, UMR1136 INRA/University of Lorraine, Labex ARB, Champenoux, France

    Francis Martin

  • Université de Lorraine, INRAE, UMR1136 “Interactions Arbres-Microorganismes”, F-54280 Champenoux, France

    Stephane Uroz

Bibliographic Information

  • Book Title: Microbial Environmental Genomics (MEG)

  • Editors: Francis Martin, Stephane Uroz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2871-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2870-6Published: 16 December 2022

  • Softcover ISBN: 978-1-0716-2873-7Published: 16 December 2023

  • eBook ISBN: 978-1-0716-2871-3Published: 15 December 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 365

  • Number of Illustrations: 17 b/w illustrations, 71 illustrations in colour

  • Topics: Microbial Genetics and Genomics, Microbiology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access