IsoLife – Stable Isotope Labelled Plant Products for the Life Sciences


May 8, 2020

IsoLife congratulates Gijs van Erven with his cum laude graduation.

October 17, 2019

IsoLife congratulates Mirjam Kabel (Food Chemistry WU) with our new, joint project.

Thesis Gijs

A new quantitative 13C-IS py-GC-MS method has been developed using U-13C Lignin as an internal standard to measure highly accurate lignin contents and acquire valuable structural information from extremely small samples (10-100 μg).

Logo NWO

This joint project was granted by NWO-TTW-OTP and entitled ‘Lignin as intrinsic tracer for gastrointestinal transit behavior and feed digestion (LiFeTracer)’. The aim of LiFeTracer is to set-up methodology for using U-13C Lignin or a recalcitrant lignin-fraction, intrinsically present in the feed, as tracer in digestion studies.

IsoLife participated in the WUR-VLAG course “Stable Isotope Methods in Nutrition Research”.

IsoLife congratulates Hiroshi Tsugawa with his article in Nature Methods.

August 26-29, 2019
Isotope course

In vivo assessment of nutrient absorption, status and metabolism can benefit a lot from the use of stable isotope methods. This course provided a solid scientific background on application of stable isotopes in nutrition research. Topics included body composition, energy expenditure, breast milk intake, vitamin/mineral bioavailability and protein digestibility.

March 28, 2019

Tsugawa et al. reported a computational approach for metabolite structure characterization using 31 uniformly 13C-labeled tissues from 12 plant species to enable metabolite classification for unknown molecules. They found 69 new metabolites currently not listed in metabolome databases.

Molecular structure of U-13C fungal cell walls unravelled.

Now also available: 0.1 atom % 13C Maize (depleted)!

July 16, 2018
Nature Communications

IsoLife‘s U-13Aspergillus fumigatus has been used to unravel the structure of fungal cell walls with solid-state NMR. The results of Kang et al. (2018) published in Nature can be used to promote the development of cell wall-targeted antifungals to combat invasive fungal infections in humans.

May 15, 2018

IsoLife recently succeeded in producing maize plants depleted in 13C as low as 0.1 atom % (D-13C Maize).

Exemption for U-13C Cannabis and U-13C Cannabinoids.

IsoLife‘s pure U-13C Lignin used as extremely accurate and precise Internal Standard.

November 17, 2017
Cannabis licentie

IsoLife has acquired an exemption from the Dutch Ministry of Health, Welfare and Sport for supplying 13C labelled cannabis plants and cannabinoids to the international research community.

September 19, 2017
Quantification of Lignin and Its Structural Features in Plant Bi

Our recently developed, pure U-13C Lignin from maize (~90%) has been used for the first time as Internal Standard for lignin quantification with Pyrolysis-GC-SIM-MS (Van Erven et al., 2017). The method was shown to be extremely accurate and proved to be a promising alternative for high-throughput quantification of lignin in biomass samples.

IsoLife sponsors the conference Jesium 2016.

IsoLife congratulates Birgit Wild with her article in Nature Scientific Reports:

September 4, 2016

The conference Joint European Stable Isotopes User group Meeting (JESIUM) was held in Ghent, Belgium on September 4 – September 9, 2016. IsoLife was sponsor of the conference and presented a poster on 13C-applications in studying changes in soil microbial processes and communities.


May 9, 2016
Scientific reports Highlights

Wild B et al., 2016. Plant-derived compounds stimulate the decomposition of organic matter in arctic permafrost soils.
Nature, Scientific Reports 6: 25607

New on IsoLife’s products list in 2016: U-13C / U-15N Fungi.

IsoLife 10 years!

December 15, 2015
Aspergillus flavus

U-13C Chitin – our first 13C fungal product – is now followed up by a large range of other fungal products.

February 8, 2015

IsoLife started on February 8, 2005 as a new, independent company with world-wide unique, stable-isotopically labelled products. Ten years later, IsoLife’s products are sold all over the world and served many scientists in the Life Sciences resulting in ~65 publications, with Science and Nature as significant highlights.

Japan Office Opened to Develop the Market in Japan for IsoLife’s Stable Isotope Labelled Phytochemicals and other Plant Products.

The project proposal “Optimizing early-life dietary carbohydrates to improve later-life metabolic health” has been granted.

February 6 , 2015
June 16, 2014

The diet in early-life can affect our physiology in later-life, with important consequences for metabolic health. Read more »

13C Inulin unravels mechanism of body weight decrease after colon fermentation.

The first rat study with U-13C Inulin has been published.

April 30, 2014

Daily intake of dietary fibers like inulin has been reported to decrease body weight. A publication inNature Communications now reveals that 13C acetate, resulting from fermentation of IsoLife‘s U-13C inulin in the colon, plays a direct role in central appetite regulation and thus in the regulation of body weight. Read more »

Nature Communications doi: 10.1038/ncomms4611.

February 26, 2014

IsoLife’s products are increasingly used in animal and human trials to clear up the complex interactions between (functional) food components and the microbial gut flora. Read more »

Applied Environmental Microbiology doi:10.1128/AEM.03799-13.

IsoLife congratulates Daniel warner with his article in Journal of Dairy Science:

ChromaDex® To Distribute IsoLife Stable Isotope Labeled Phytochemicals And Plant Products.

July 8, 2013

For the first time, 13C-maize has been used to study the passage kinetics of maize silage components through the gastrointestinal tract of dairy cows. Journal Dairy Science 96 :1–15.  

January 22, 2013

IsoLife bv announced today it has signed a distribution agreement with ChromaDex®

Corporation (OTCQB: CDXC), an innovative natural products company. IsoLife’s 13C- and 15N-labeled plant products will be distributed in the USA, Canada, Brasil and Korea as tracers and internal standards for food & beverage, nutraceutical, pharmaceutical, cosmeceutical and environmental sciences research. Read more »

NanoSIMS reveals 13C-carbon tranfer between symbiotic organisms.

First results on sulfur-containing metabolite-targeted analysis using LC-FT-MS and U-13C Onion bulb.

September 21, 2012
September 9, 2012

Now also available: U-13C and U-15N Chlorella vulgaris.

Food for Thought: The meaningful role of stable isotopes in health-related food research.

September 1, 2012
Chlorella by Microscope

Stable isotope labelled Chlorella vulgaris is used in food studies. It is an attractive potential food source because of its high protein concentration: 45% (Wikipedia).

June 14, 2012

Stable isotopes are playing an increasingly crucial role in state-of-the-art applications in health-related science and technology.

This overview in Food for Thought of IsoLife‘s current research projects involving stable isotopes in food applications will explain this new development. Read more »

IsoLife proudly presents BroccoCress grown from uniformly 13C-labelled seeds.

IsoLife sponsors the conference ‘BASIS 2012′.

March 19, 2012

The broccoli sprouts will be used in clinical studies on bioavailablity.

March 1, 2012
Benelux Association Stable Isotope Scientists

After meeting in Brugge in 2011, the 6th annual meeting of the Benelux Association for Stable Isotope Scientists (BASIS) 2012 will be held in

Nijmegen, The Netherlands, in “Forum de Ganzenheuvel” in the city center, on Thursday and Friday, April 12 and 13, 2012.

IsoLife sponsors the conference ‘MR in FOOD 2012’.

IsoLife congratulates Toby Kiers with her article in Science:

November 16, 2011

The 11th International Conference on the applications of Magnetic Resonance in Food will be held in Wageningen, The Netherlands on June 26 – June 29, 2012. IsoLife will sponsor the

conference and will present posters on 13C-applications in tracing foods and in quantitative mass spectrometry.

September 15, 2011

IsoLife proudly presents the first uniformly 13C-labelled tree species!

IsoLife sponsors the conference ‘Metabolomics 2011’.

August 15, 2011
June 27, 2011
Metabolomics congress

The 7th International Conference of the Metabolomics Society will be held in Cairns, Australia on June 27 – June 30, 2011. IsoLife was sponsor of the

 conference and presented a poster on 13C-applications in metabolomics.

IsoLife sponsors the conference ‘Ecology of Soil Microorganisms 2011’.

IsoLife publication:’No de novo sulforaphane biosynthesis in broccoli seedlings’.

May 2, 2011

The Conference ‘Ecology of Soil Microorganisms held in Prague on April 27 – May 1, 2011 was a well-organised and successful meeting.

Several contributions showed that 13C-applications do extend the possibilities to identify active soil microbes assimilating carbon from different substrates in complex ecosystems. For those who missed it: download our poster presentation here.

December 17, 2010

The isothiocyanate sulforaphane, present in significant amounts in broccoli (Brassica oleracea L.) seedlings in the form of its precursor glucoraphanin, has been identified as an inducer of quinine reductase, a phase-II detoxification enzyme known for its anticarcinogenic properties. Here, we unambiguously demonstrate in that sulforaphane is not biosynthesised de novo during the first week of seedling development. Both 12C (99 atom%12C) and 13C (98 atom% 13C) broccoli seeds were produced and subsequently germinated and grown either in a 13CO2 or a 12CO2environment. Afterwards, the labelling degree of sulforaphane in seeds and in seedlings was analysed by HPLC–MS. We conclude that sulforaphane exclusively originates from seed reserves and that de novo biosynthesis is not detectable in broccoli seedlings. Our findings have been published in Food Chemistry (for further details see Applications). Ask a reprint.

Steering and control of plant quality.

Trace gas detector central in large EU project Ultragas.

Januari 11, 2010

A challanging new project was granted on the steering and control of plant quality within the Biotech Systems Platform.

December 9, 2009
go gebundeld lettercontouren

The trace gas detector developed at Radboud University Nijmegen plays a central role in a 4,2 Meuro EU-project. The innovative measuring technique will be applicated in fruit storage, greenhouse crops, and in medical diagnostics.

IsoLife is one of the partners to further develop this high-tech detector for use in her labeling facility. This project was made possible by a financial

contribution of the European Union. Here is invested in your future:

European flag

Intecol 2009.

Stable Isotopes Support Functional Ingredients Research.

August 25, 2009

The Intecol congress held in Brisbane on August 15 – 21, 2009 has been a very successful event. Interesting discussions with participants, nice temperatures, and good foods & drinks, have yielded new valuable ideas on how to use stable isotope labelled higher plants and plant components in ecological sciences.

June 1, 2009
Food Marketing & Technology

Interview in Food Marketing & Technology about the use of stable isotopes in food research.
Read more »

A novel method for the quantification of quinic acid in food products.

Prize winning publication with IsoLife‘s 13C-potato starch.

May 27, 2009

Publication of “A novel method for the quantification of quinic acid in food using stable isotope dilution analysis”, based on the use of IsoLife‘s U-13C Quinic acid as an internal standard.

May 22, 2009

The scientific paper “Linking phylogenetic identities of bacteria to starch fermentation in an in vitromodel of the large intestine by RNA-based stable isotope probing” won the prestigious TIFN publication prize. Read more »

IsoLife receives the TechnoPartner Certificate.

Healthy food: Potato products with low caloric values and high dietary fiber contents.

May 14, 2009
TechnoPartner Certificate

The Dutch minister of Economic Affairs Maria van der Hoeven and ABN AMRO have awarded IsoLife’s highly innovative R&D plans with the TechnoPartner Certificate. The certificate acknowledges the courage, knowledge, and perseverance to further develop her innovative initiatives to commercial successes.

March 1, 2009

Potatoes are a good, natural source of carbohydrates, energy, dietary fiber, minerals, and vitamins.13C-labeled potatoes are extremely helpful to investigate the caloric properties and the behavior of functional components of specific potato varieties in a normal daily diet. For this purpose, IsoLife has produced several stable isotope labelled potato varieties during recent years. One of the projects – co-financed by ‘Samenwerkingsverband Noord-Nederland’ and performed by IsoLife in co-operation with the partners HZPC, Aviko and the University Medical Center Groningen – investigated whether the caloric value of potatoes and potato products could be (further) decreased by specific procedures in potato breeding or product processing.

HZPC Holland BV is a breeding company and a trade company for the development and sales of innovative potato varieties in the potato supply chain with focus on potatoes for consumption and potatoes for French fries, chips and the food chain.
AVIKO BV is the largest Dutch producer of potato products with focus on health aspects.
The ‘Center for Medical Biomics’ (UMCG), the department of professor Roel Vonk, has contributed in areas like digestion, fermentation, and metabolism of potato components in relation to human health. The UMCG has co-ordinated the large EU-research project EUROSTARCH

The results, based on detailed experiments with 13C-labelled potato products, show that the caloric value of potato products can indeed be (further) decreased through changes in their composition.

EU logo

This project was co-financed by the European Community, the European Regional

SNN logo

Development Fund, and the “Samenwerkingsverband Noord-Nederland (SNN)”, a joint agreement between the provinces of Drenthe, Groningen and Fryslân. EZ/KOMPAS under number AA099.

Development of U-13C Cannabinoids and U-13C Hemp oil fatty acids.

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September 1, 2008

IsoLife BV, Prisna BV and Bedrocan BV have joined forces to develop a new high-quality hemp oil and an innovative set of reference standards for mass spectrometry, including 13C-labelled cannabinoids and hemp oil fatty acids.

June 7, 2008
FEM Business and Finance

IsoLife BV has been identified by FEM Business Tech 25 as one of 25 young, innovative companies who ‘shake the market with brilliant ideas’.

Methane emission from plants: Yes they do! No they don’t!

The first uniformly uniformly 13C-labelled tomatoes (Lycopersicon esculentum Mill. cv ‘MicroTom’) were harvested.

April 27, 2007

In 2006, Nature published a sensational article by Keppler et al, who claimed that plants would be able to emit methane under aerobic conditions. A Dutch consortium consisting of IsoLife, Plant Research International, Plant Dynamics, Nijmegen Radboud University, Utrecht University tried to reproduce their results using a novel technique with uniformly 13C-labelled plants. We found methane emissions that were between 0.1% and 0.3% of the rates reported by Nature. Apparently, the textbooks do not have to be rewritten. Our findings have been published in New Phytologist (for further details see Application; for more news read the News commentaries). Ask a reprint.

July 20, 2006

We are proud to have served our customers with mature, uniformly (~98% 13C) labelled tomatoes.

First results published on U-13C Starch using RNA-SIP

Wageningers produceren planten met louter zware isotopen

December 1-3, 2005

Kovatcheva-Datchary et al. published their results of research performed at the Wageningen Centre of Food Science (WCFS; with U-13C Starch using the RNA-SIP-technique on

the congress “Biomedicine in the Post-Genomic Era” in Mexico. They concluded that “RNA-SIP has the potential to elucidate microbial structurefunction relationships in the human colon, e.g. regarding the fermentation of dietary relevant carbohydrates”. For more details download the WCFS poster.

April 2, 2005

Zware planten
Twee Wageningse
biologen kweken planten op in een hermetisch gesloten ruimte. Zo kunnen ze planten laten groeien op voeding naar keuze die geheel voorzien is van zware isotopen. ‘Dat opent een heel nieuwe wereld van onderzoek.’
bron: NRC Handelsblad Wetenschap & Onderwijs – Sander Voormolen

IsoLife wins the Dutch National BioPartner Masterclass Award 2004

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February 3, 2005
News Highlight IsoLife

IsoLife’s businessplan received the BioPartner Masterclass Award 2004. The plan was judged by a jury consisting of representatives from industry, the financial world, and BioPartner.

The founders of IsoLife, Ton Gorissen and Ries de Visser, received the price from Chris Buijink, Director-General Enterprise and Innovation at the Dutch Ministry of Economic Affairs.